Construct size (kb): 4.5

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSP65
Intact vector size: 3.010
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Polylinker sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
marker(s): ampR
promoter: SP6
replicon: pMB1
terminator: none
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: IFI16
Other gene symbols: IFNFIP1
Genomic copy number: unique
Gene name: interferon, gamma-inducible protein 16
Contains complete coding sequence?: U
Chromosome: 1; Localization: 1 q22
Tissue: T lymphocyte, activated
Type of DNA: cDNA
Insert 5' end: Modification: linker
Insert 3' end:
Insert size (kb): 1.53
Cross references: DNA Seq. Acc.: M63838

Insert lengths(kb): 1.529999971389771

Tissue: T lymphocyte, activated

Gene product: interferon, gamma-inducible protein 16(interferon gamma-inducible protein 16) [IFI16]

Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.5; HindIII--3.4, 1.1; PstI--3.0, 1.5; PvuII--2.5, 1.2, 0.7; SacI--4.5.

The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.32; HindIII--0.79; PvuII--1.29.

Corresponds to nt 403-1933 of the published sequence (encoding aa 135-644).

Detects a transcript of 2.7 kb in lymphoid cells.