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pJ819 [J819] plasmid in E. coli
pJ819 [J819] plasmid in E. coli
規格:
貨期:
編號:B244884
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pJ819 [J819] plasmid in E. coli
商品貨號 B244884
Designations pJ819 [J819] plasmid in E. coli
Species Homo sapiens, human
Depositors EH Chang
Vector
Construct size (kb): 8.2
Insert
DNA: genomic
Insert lengths(kb): 3.8
Tissue: liver
Gene product: Kirsten rat sarcoma 1 viral (v-Ki-ras1) oncogene homolog, processed pseudogene [KRAS1P]
Insert Size (kb) 3.800
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
The insert contains BalI, AccI (3), BglII (2), SacI (2), EcoRI (2), and XbaI sites.
TaqI RFLP best detected using a 1.1 kb BglII/HindIII segment of the insert, because the 3.8 kb HindIII insert contains a repetitive sequence.
Enzyme(s) not detecting polymorphism (in a panel of 5): BamHI, BglII, EcoRI, HindIII, HincII, MspI, PstI, RsaI.
Enzymes not detecting polymorphism: PstI, EcoRV, BamHI, BglII, MspI, EcoRI, HindIII, PvuII (10 unrelated Caucasians).
Using this probe on TaqI digested human DNA, three RFLP alleles were detected. A1 (22.00 kb), A2 (18.00 kb), A3 (19.00 kb). Frequency of detection of alleles: A1/A2/A3 = 0.48/0.51/0.01
References

Chang EH, et al. Human genome contains four genes homologous to transforming genes of Harvey and Kirsten murine sarcoma viruses. Proc. Natl. Acad. Sci. USA 79: 4848-4852, 1982. PubMed: 6289320

Blanche H, et al. A fragment of the human c-Ki-ras1 pseudogene (HMG9 gene symbol KRAS1P), localized to 6p12-p11, detects 3 allele, RFLP. Nucleic Acids Res. 16: 1652, 1988. PubMed: 2894641

Barbara Weiffenbach, personal communication

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