in another host, produces protein dUTP pyrophosphatase

Construct size (kb): 3.5

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pTZ19R
Intact vector size: 2.900
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC19, Escherichia coli lacZalpha, pBR322, f1
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T7
replicon: pMB1, f1
terminator: none
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: DUT*
Genomic copy number: multiple
Gene name: dUTP pyrophosphatase (dUTPase)
Contains complete coding sequence?: Y
Chromosome: 15; Localization: 15, cR
Type of DNA: cDNA
Insert end: Modification: EcoRI adapters
Insert end: Modification: EcoRI adapters
Insert size (kb): 0.6
Cross references: DNA Seq. Acc.: M89913

Insert lengths(kb): 0.6000000238418579

Gene product: dUTP pyrophosphatase [DUT*]

Target Gene: dUTP pyrophosphatase

Restriction digests of the clone give the following sizes (kb): HindIII--3.5; XmnI--2.4, 1.1; SmaI--3.3, 0.15; EcoRI--2.9, 0.6; PstI--3.5.

The insert contains the following restriction sites (nt from the 5' end): SphI--17, 86; SmaI--134.

Contains the complete coding sequence, and corresponds to nt 1-545 of the database record.

The sense strand is packaged.

pEM301 (ATCC 79032/3) and pEM302 (79034/5) differ only in the orientation of the insert.