Distribution host: Escherichia coli HB101 (ATCC 33694)

Size (kb): 6.9270000457763670

DESCRIPTION OF VECTOR:
Intact vector size: 6.927
Type of vector: plasmid
Cloning sites: XhoI SalI HindIII PstI SmaI BamHI SpeI XbaI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS415
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): LEU2, ->, 200-2446
terminator: CYC1, ->, 3128-3388
MCS: XhoI...XbaI, ->, 3388-3452
promoter for expression: GPD, <-, 3452-4132
marker(s): ampR, <-, 5379-6309
replicon: CEN6/ARSH4, ->, 6141-6927

Vector: p415 GPD (plasmid)

Promoters: Promoter for expression GPD

Construction: pRS415

Marker(s):LEU2,ampR

Construct size (kb): 6.927000045776367

Features: marker(s): LEU2
marker(s): ampR
promoter for expression: GPD
replicon: CEN6/ARSH4
MCS: XhoI...XbaI
terminator: CYC1

YX-type (expression) shuttle vector

vector with low copy number

Restriction digests of the clone give the following sizes (kb): SacI/XbaI--6.2, 0.7; EcoRI--4.6, 2.3; BamHI--6.9.

Low copy number shuttle expression vector.

Temperature: 37.0°C

component of:ATCC 87669