Size (kb): 4.1519999504089360

Vector: pMA200U (phagemid)

Promoters: Promoter lambda PL

Construction: pCE30 (ATCC 37830), pTZ18U

Marker(s):ampR

Construct size (kb): 4.151999950408936

Features: marker(s): ampR
promoter: lambda PL, lambda PR
replicon: pMB1, f1
repressor gene: cI857

expression vector

vector containing primer sites useful for sequencing

vector permitting production of single-stranded DNA

Restriction digests of the clone give the following sizes (kb): BamHI--4.3; BamHI/EcoRI--4.3; BglI--3.0, 1.3.

Upon infection with M13KO7 (ATCC 37468), an M13-sensitive host strain containing a pMA200U derivative produces packaged single-stranded plasmid DNA complementary to the M13 universal primer.

If the insert is oriented correctly for transcription from the lambda promoters, the single-stranded plasmid DNA will contain the coding strand.

Expression vector encoding cI857. Contains primer sites useful for sequencing and permits production of ssDNA.

Constructed by replacing the 1.118 kb BglI fragment of pCE30 with the analogous 1.27 kb fragment of pTZ18U.

Temperature: 30.0°C