| 產(chǎn)品名稱(chēng) |
c8543 |
| 商品貨號(hào) |
B243592 |
| Designations |
c8543 |
| Species |
Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors |
L Riles, MV Olson |
| Vector |
Construct size (kb): 45.29999923706055 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: SuperCos 1
Intact vector size: 7.600
Type of vector: cosmid
Cloning sites: BamHI
Polylinker sites: EcoRI NotI BamHI NotI EcoRI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, neoR, G418R
promoter: T3, T7
replicon: pMB1, SV40
Cross references: |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Saccharomyces cerevisiae
Gene symbol: CDC3
Contains complete coding sequence?: Y
Chromosome: XII; Localization: XII R, 225 cM from CEN
Genome: Saccharomyces cerevisiae
Gene symbol: REC102
Contains complete coding sequence?: Y
Chromosome: XII
Genome: Saccharomyces cerevisiae
Gene symbol: MID2
Gene name: mating process protein
Contains complete coding sequence?: Y
Chromosome: XII
Strain: AB972 (ATCC 76269)
Type of DNA: genomic
Insert end:
Insert end:
Insert size (kb): 37.7
Cross references: Insert lengths(kb): 37.70000076293945 Gene product: mating process protein [CDC3] |
| Insert Size (kb) |
37.700 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: frozen, uncharacterized |
| Comments |
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. |
| Classification |
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
