in another host, produces protein interleukin 9 receptor

Construct size (kb): 7.400000095367432

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pRc/RSV
Intact vector size: 5.100
Type of vector: phagemid
Vector end: BstXI
Vector end: BstXI
Cloning sites: HindIII SpeI BstXI NotI XbaI
Polylinker sites: HindIII KpnI SacI BamHI SpeI XmaIII BstXI EcoRI PstI EcoRV
BstXI NotI XhoI SphI NsiI XbaI
Construction: pUC19, RSV, bovine growth hormone
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, neoR, G418R
promoter: RSV LTR
replicon: pMB1, M13
terminator: BGH polyadenylation
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: IL9R
Gene name: interleukin 9 receptor
Contains complete coding sequence?: Y
Tissue: megakaryoblastic leukemia Mo7E cell line
Type of DNA: cDNA
Insert end: BstXI
Insert end: BstXI
Insert size (kb): 2.3
Cross references: DNA Seq. Acc.: M84747

Insert lengths(kb): 2.299999952316284

Tissue: megakaryoblastic leukemia Mo7E cell line

Gene product: interleukin 9 receptor [IL9R]

Target Gene: interleukin 9 receptor

Restriction digests of the clone give the following sizes (kb): HindIII/NotI--5.4, 2.5; BamHI--3.0, 2.85, 2.1; PstI--6.4, 1.5; SmaI--5.2, 2.75; EcoRI--4.4, 3.5.

The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.08, XbaI--0.33; SfiI--1.17; SmaI--1.33; SacI--1.34, 1.57, 2.25.

Contains the complete coding sequence (nt 180-1748) plus 5' and 3' untranslated regions.

The 5' end of the insert is adjacent to the unique HindIII and SpeI sites of the MCS; the 3' end of the insert is adjacent to the unique NotI site of the MCS.