Size (kb): 7.3359999656677250

Vector: pCu425CUP1 (phagemid)

Promoters: Promoter for expression CUP1

Construction: pRS425, CUP1 promoter

Marker(s):LEU2,ampR

Construct size (kb): 7.335999965667725

Features: marker(s): LEU2
marker(s): ampR
promoter for expression: CUP1
replicon: 2 micron
replicon: f1
replicon: pMB1
MCS: SpeI...XhoI
terminator: CYC1

cloning vector

Restriction digests of the vector give the following sizes (kb): SacI--7.3; SacI/SpeI--7.0, 0.26.

Gene CUP1 is the most potent for copper induction. The CUP1 promoter can be used to creat a series of versatile vectors to allow its utilization to control the expression of heterologous genes in S.cerevisiae.

component of:ATCC 87778