Construct size (kb): 49.0

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: Charon 4A
Intact vector size: 46.000
Type of vector: phage
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
promoter: none
replicon: lambda
terminator: none
Cross references:

DNA: genomic

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GC
Genomic copy number: unique
Gene name: group-specific component (vitamin D binding protein)
Contains complete coding sequence?: N
Chromosome: 4; Localization: 4 q12-q13
Tissue: fetal liver (ATCC 37333)
Type of DNA: genomic
Insert end: HaeIII or AluI Modification: EcoRI linker
Insert end: HaeIII or AluI Modification: EcoRI linker
Insert size (kb): 18.0
Cross references: DNA Seq. Acc.: L10641
Nucleotides ~1-18000 of the insert correspond to
nucleotides ~37000-55136 of L10641.

Insert lengths(kb): 18.0

Tissue: fetal liver (ATCC 37333)

Gene product: group-specific component (vitamin D binding protein)( vitamin D binding protein, vitamin D-binding alpha-globulin, group specific component) [GC]

Restriction digests of the clone give the following sizes (kb): EcoRI--19.5, 11, 9.8, 3.5, 3.3, 1.4; HindIII--19, 14, 6, 4.4, 4, 2, 0.4; BamHI--17, 11, 7.5, 5.8, 3.9, 3, 1.6, 0.5; BglII-->23, 5.8, 5, 2.15, 1.95, 1.9, 1.8, 1.4, 0.7, 0.4;

SstI-->23, 15, 4, 3.8, 1.9.

The insert contains the following restriction site (approximate kb from the 5' end): EcoRI--1.4, 4.7, 14.5; HindIII--6.6, 11.0, 15.0, 15.4, 17.4; SacI--14.6, 18.8.

Begins in intron 11 and extends into the 3' flanking region.

ATCC 95668/95669 and 95670/95671 overlap by 11.9 kb and share an EcoRI fragment of 3.3 kb.

Contains Alu repeats, L1 (Kpn) repeats, and a THE repeat.