in another host, produces protein thymidine kinase 2, mitochondrial

Construct size (kb): 4.775000095367432

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pcD
Intact vector size: 3.000
Type of vector: plasmid
Cloning sites: XhoI BamHI
Polylinker sites:
Construction: pBR322, SV40
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
enhancer: SV40
marker(s): ampR
promoter: SV40 early, late
replicon: pMB1, SV40
terminator: SV40 late
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: TK1
Genomic copy number: unique
Gene name: thymidine kinase 1, soluble
Contains complete coding sequence?: Y
Chromosome: 17; Localization: 17 q23.2-q25.3
Type of DNA: cDNA
Insert 5' end: Modification: GC
Insert 3' end: Modification: AT
Insert size (kb): 1.42
Cross references: DNA Seq. Acc.: K02581

Insert lengths(kb): 1.419999957084656

Gene product: thymidine kinase 1, soluble [TK1]

Alleles: A1, A2, B1, B2, B3

Target Gene: thymidine kinase 2, mitochondrial

Restriction digests of the clone give the following sizes (kb): BamHI--3.1, 1.6, 0.23; BamHI/SmaI--3.25, 1.40, 0.3; BglI--2.9, 1.25, 0.6, 0.38; HindIII--3.4, 1.55; XhoI--3.25, 1.90.

The insert contains the following restriction sites (approximate kb from the 5' end): SmaI--0.11; BglI--0.31, 0.65; HindIII--0.96; BamHI--1.34.

Although the GenBank K02581 gives the gene symbol for this sequence as TK2, this is the cDNA derived from the genomic sequence M15205, the gene for thymidine kinase 1 (TK1).

Contains the complete coding sequence (nt 58-762).

A 1.25 kb SmaI/BamHI fragment has been used as a hybridization probe. It detects a 3.0 kb HindIII fragment in chickens.