Size (kb): 4.8000001907348630

Vector: pSLF104 (plasmid)

Promoters: Promoter adh

Marker(s):ampR,sup3-5

Construct size (kb): 4.800000190734863

Features: marker(s): ampR
marker(s): sup3-5
promoter: adh
repressor gene: tet
MCS: HindIII...EcoRI

YI-type (integrating) shuttle vector

Restriction digests of the clone give the following sizes (kb): PstI--2.8, 1.4, 0.58; BamHI--2.9, 1.9; EcoRI--3.6, 1.2.

The vector contains the marker sup3-5, which suppresses ade6-704, and the tet repressor gene under control of the S. pombe adh promoter.

When integrated into a strain of genotype h- ade6-704 leu1-32 ura4-D18, the resulting fission yeast strain, FY191 (ATCC ) produces a constitutive tetracycline repressor protein.

Temperature: 37.0°C

component:ATCC 201437

component of:ATCC 87685