Size (kb): 5.0999999046325680

Vector: p309 (plasmid)

Promoters: Promoter lac

Construction: pUC18, aph, MSV promoter and enhancer

Marker(s):G418R,ampR

Construct size (kb): 5.099999904632568

Features: insert detection: lacZ'
marker(s): ampR, G418R
promoter: lac, MSV
replicon: pMB1
enhancer: MSV

expression vector

shuttle vector

vector permitting construction of fusion proteins

XbaI, SmaI, SstI and EcoRI appear in both the polylinker and the aph cassette.

Restriction digests of the clone give the following sizes (kb): HindIII--4.8; EcoRI--4.7, 0.2.

An Escherichia coli/eukaryotic cell line shuttle vector containing lacZ and multiple cloning sites of pUC18 and the aph (neoR) gene under the control of murine sarcoma virus promoter and enhancer.

The PvuII at position 628 of pUC18 was changed to BglII. A cassette containing the aph gene under control of murine sarcoma virus promoter and enhancer was inserted at the BglII site. Aph is transcribed in the opposite direction of lacZ.

Temperature: 37.0°C