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Vaccinia virus ts mutant
Vaccinia virus ts mutant
規格:
貨期:
編號:B240138
品牌:Mingzhoubio

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產品名稱 Vaccinia virus ts mutant
商品貨號 B240138
Classification Poxviridae, Orthopoxvirus
Agent Vaccinia virus ts mutant
Strain IHD-W Dts95 (Dales isolate 9312)
Applications
The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U21.
Positive for Mycoplasma No
Biosafety Level 2 CDC BMBL recommends vaccination.
Isolation
derived from existing strain
Product Format frozen
Storage Conditions -70°C or colder
Comments
Not described in the original publication, but presumed to have been selected after mutagenesis with nitrosoguanidine. The non-permissive incubation temperature is 39.5oC. This was not assigned a Dales EM category. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U21.
Effect on Host
Yes, Cytopathic effect: Rounding and sloughing of infected cells
CPE: enlargement, plaques with rounding and eventual sloughing
Recommended Host

Recommended Host: BSC-40 (ATCC CRL-2761
Growth Conditions
Temperature: 31.0°C
Duration: 1-3 days; For best results, cells should be 24 to 48 hours old and 80-90% confluent at time of infection [not 100% confluent].
Name of Depositor S Dales, RC Condit, S Dales
Special Collection NCRR Contract
Source
derived from existing strain
References

Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807

Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746

This mutant strain was derived by Samuel Dales from the IHD-W strain of Vaccinia (ATCC VR-1441) which in turn was derived from the IHD-J strain.

cell enlargement, plaques with rounding and eventual sloughing of cells

Biosafety in Microbiological and Biomedical Laboratories 4th ed.; U.S. Department of Health and Human Services ;1999.

Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570

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