in another host, produces protein pituitary-specific transcription factor (growth hormone factor 1)

Construct size (kb): 5.599999904632568

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pRSVcat
Intact vector size: 5.100
Type of vector: plasmid
Vector end: XmaI
Vector end: XbaI
Cloning sites: BamHI HindIII
Polylinker sites:
Construction: pSV2-betaG, Tn9, RSV
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
enhancer: RSV
marker(s): ampR, cmLR
promoter: RSV LTR
replicon: pMB1
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PIT1
Genomic copy number: unique
Gene name: pituitary-specific transcription factor (growth hormone
factor 1)
Contains complete coding sequence?: Y
Chromosome: 3; Localization: 3 p11
Tissue: pituitary
Type of DNA: cDNA
Insert 5' end: XmaI
Insert 3' end: XbaI
Insert size (kb): 1.0
Cross references: DNA Seq. Acc.: X72215

Insert lengths(kb): 1.0

Tissue: pituitary

Gene product: pituitary-specific transcription factor (growth hormone factor 1) [PIT1]

Target Gene: pituitary-specific transcription factor (growth hormone factor 1)

Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 2.1, 0.4, 0.24; PstI--2.5, 1.8, 1.0; PvuII--5.4; SphI--4.6, 0.9; SmaI/XbaI--4.4, 1.1.

Insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--0.11, 0.56; PstI--0.6; PvuII--0.38, 0.54; SphI--0.62.

Contains the complete coding sequence plus portions of the 5' and 3' untranslated regions.

This clone varies from other published sequence(s): GenBank records X62429, D01114. The aa sequence differences determined by GenBank X72215 are: Q4R, D227Y.

The ends of the PIT1-specific portions of the primers used to amplify this sequence correspond to nt 56-1046 of GenBank record D01114. In this record, the coding region extends from nt 126-1001.

Insert was amplified using the following primers: 5'CCCCCCGGGAGACAGTAATATAATAA 3'(5' untranslated region) and 5'CCCTCTAGAGAAGGAATGAAACGGG 3' (3' untranslated region).