Construct size (kb): 4.199999809265137

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GAD1
Other gene symbols: GAD
Genomic copy number: unique
Gene name: glutamate decarboxylase 1 (brain)
Contains complete coding sequence?: U
Chromosome: 2; Localization: 2 q31
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.2
Cross references: DNA Seq. Acc.: M85674; EST: EST02192; Primer: M13 Forward

Insert lengths(kb): 1.200000047683716

Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)

Gene product: glutamate decarboxylase 1 (brain)(glutamic acid decarboxylase) [GAD]

Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.2; PstI--4.2; HindIII--4.2.

EST02192 matches GenBank accession HUMGAD with 100% identity over the sequence range: 227-14.