Distribution host: Escherichia coli BR2943

Size (kb): 3.5000000000000000

DESCRIPTION OF VECTOR:
Intact vector size: 3.500
Type of vector: plasmid
Cloning sites:
Polylinker sites: EcoRI SalI BamHI BglII BssHII PvuI KpnI PstI PvuII ClaI NruI
SacI XhoI BglI HindIII
Construction: pVEX1191
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: P1
restriction site: PstI
marker(s): strR, spcR
restriction site: NotI
MCS: HindIII...EcoRI, ->
restriction site: NotI
other: loxP, ->
restriction site: PstI

Vector: pVEX1211 (plasmid)

Construction: pVEX1191

Marker(s):spcR,strR

Construct size (kb): 3.5

Features: marker(s): strR, spcR
other: loxP
replicon: P1
MCS: HindIII...EcoRI
restriction site: NotI
restriction site: PstI

host modification

vector for other uses

vector permitting positive selection for integration

Restriction digests of the clone give the following sizes (kb): BamHI--3.6; EcoRI--3.6; PstI--2.1, 1.1, 0.35.

To be used with pVEX2211 or pVEX2212 (ATCC 77422, 77423).

The vector is used to clone a sequence flanking the target to be deleted. The vector will be integrated by homologous recombination at the cloned sequence when spectinomycin selection is imposed in a host not expressing repA.

The deletion derivative retains one loxP site and the cmlR marker. The circularized, deleted sequence is lost from cells not expressing repA or pir.

In the presence of Cre recombinase (from E. coli EKA133 ATCC 47071), double cointegrates [pVEX1211(pVEX1212) and pVEX2211(pVEX2212)] provide two loxP sites for site-specific recombination that deletes the intervening target sequence.

Component of a vector-mediated excision system for generating in vivo deletions of large bacterial genomes, self-transmissible plasmids or temperate E. coli bacteriophages.

Differs from pVEX1212 (ATCC 77421) only in the orientation of the polylinker.

Temperature: 37.0°C