Construct size (kb): 16.5

DNA: genomic

Insert lengths(kb): 5.800000190734863

Gene product: uridine monophosphate synthetase( UMP synthase, orotate phosphoribosyltransferase, orotidine 5'-phosphate decarboxylase, orotate phosphoribosyltransferase 1) [URA3]

Restriction digests of the clone give the following sizes (kb): EcoRI--6.8, 4.4 (doublet), 2.3, 0.4; EcoRI/HindIII--4.4 (doublet), 2.3, 1.6, 1.4 (doublet), 1.25, 0.75, 0.4; BamHI--uncut; BglI--11.0, 5.0, 2.4; EcoRV--7.2, 3.9, 3.4, 2.2, 1.0, 0.3;

PstI--9.6, 4.3, 2.6, 1.0 (doublet).

This plasmid complements the pyrF mutation in E. coli.

A 6.2 kb EcoRI fragment contains 377 bp of vector sequence and all of the insert except for 50 bp. The gene is in a 2.8 kb EcoRI/SacI fragment.