Distribution host: Escherichia coli HB101 (ATCC 33694)

Size (kb): 6.6700000762939450

DESCRIPTION OF VECTOR:
Intact vector size: 6.670
Type of vector: plasmid
Cloning sites: NotI, XbaI
Polylinker sites:
Construction: pBluescript KS+
Host range: mammalian cells; Escherichia coli
Features (with orientation and position when available):
other: internal ribosome entry sequence (IRES), ->
reporter group: lacZ, ->
replicon: pMB1
marker(s): ampR

Vector: pW60 (plasmid)

Construction: pBluescript KS+

Marker(s):ampR

Construct size (kb): 6.670000076293945

Features: marker(s): ampR
other: internal ribosome entry sequence (IRES)
replicon: pMB1
reporter group: lacZ

reporter construct beta-galactosidase beta-D-galactosidase

Restriction digests of the clone give the following sizes (kb): EcoRI--3.6, 3.1; HindIII--6.4, 0.3.

Plasmid expressing beta-galactosidase. Foreign genes can be cloned upstream of the IRES/reporter gene, allowing production of a dicistronic messenger RNA in mammalian cells.

IRES/reporter insert can be released by XhoI.

Temperature: 37.0°C