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pKLS12
pKLS12
規(guī)格:
貨期:
編號(hào):B237265
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pKLS12
商品貨號(hào) B237265
Designations pKLS12
GenBank Number

U07617

Species Mus musculus, mouse
Depositors M Barbacid, K Suen
Vector
Construct size (kb): 5.300000190734863
Insert
DNA: cDNA
Insert lengths(kb): 2.361999988555908
Gene product: growth factor receptor-bound protein 2 (C. elegans sem-5 related) [Grb2]
Insert Size (kb) 2.362
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): PvuII--2.5, 1.5, 1.3; PstI--4.1, 1.3; KpnI--4.1, 1.2; EcoRI/XhoI--3.0, 2.3; BglI--3.7, 1.2, 0.4.
Transcription from the T3 promoter gives the sense strand. The plasmid can be linearized with XhoI.
The insert contains the following restriction sites (approximate kb from the 5' end): BglI--2.16; EcoRV--2.29; KpnI--1.21; NcoI--2.09, 2.18; PstI--1.30; PvuII--1.05; SacI--0.01.
The protein differs from the human GRB2 sequence only at aa 154, where the murine protein has Met instead of Val.
Detects transcripts of 3.8 kb and 1.5 kb in the following mouse tissues: lung, heart, testis, muscle, cerebellum, brain, kidney, spleen, ovary, thymus and at low levels in the liver.
Constructed by ligation of a 5' 155 bp EcoRI-AflII fragment, amplified from a kidney cDNA library, and a 3' 2,206 bp AflII-XhoI fragment, isolated from a brain cDNA library.
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Suen KL, et al. Molecular cloning of the mouse grb2 gene: differential interaction of the Grb2 adaptor protein with epidermal growth factor and nerve growth factor receptors. Mol. Cell. Biol. 13: 5500-5512, 1993. PubMed: 7689150

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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