Distribution host: Escherichia coli DH5

Size (kb): 5.0349998474121090

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: sBSL98
Intact vector size: 3.100
Type of vector: phagemid
Vector end: mLuI
Vector end: mLuI
Cloning sites:
Polylinker sites: KpnI ApaI XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI
SpeI XbaI NotI/EagI BstXI SacII SacI mLuI
Construction: pBluescript
Host range: Escherichia coli
Features (with orientation and position when available):
MCS: KpnI...SacI mLuI, ->
MCS: mLuI KpnI...SacI, ->
replicon: pMB1
marker(s): ampR, <-
replicon: f1, <-
Cross references:

Vector: pBSL130 (phagemid)

Promoters: Promoter for in vitro transcription T7 (phi10)

Construction: pBluescript

Marker(s):ampR,spcR,strR

Construct size (kb): 5.034999847412109

Features: marker(s): ampR
promoter for in vitro transcription: T7 (phi10)
replicon: f1
replicon: pMB1
MCS: KpnI...SacI MluI
MCS: MluI KpnI...SacI
coding sequence: aadA (spcR, strR)

contains easily purifiable cassette(s) for construction omega-aadA1

Restriction digests of the clone give the following sizes (kb): MluI--3.0, 2.0; EcoRI--2.8, 2.1; BamHI--2.8, 2.1.

Plasmid containing an easily purified spcR (strR) cassette (flanked by direct repeats of the a multiple cloning sequence) for use in construction.

Temperature: 37.0°C