Size (kb): 7.5999999046325680

Vector: p2UGCAT (plasmid)

Promoters: Promoter CYC1

Construction: p2UG, CAT

Marker(s):URA3,ampR

Construct size (kb): 7.599999904632568

Features: marker(s): ampR, URA3
promoter: CYC1
replicon: pMB1, 2 micron
enhancer: GRE

YE-type (episomal) shuttle vector

expression vector

reporter construct

shuttle vector

vector with sequences for protein export

Restriction digests of the clone give the following sizes (kb): BamHI--7.6, EcoRI--2.2 (doublet), 1.7, 1.4; KpnI--7.6.

The URA3 marker is from S. cerevisiae but complements the ura4 allele from Sc. pombe.

Expression of chloramphenicol acetyltransferase is hormone-inducible in both S. cerevisiae and Sc. pombe hosts, in the presence of a glucocorticoid receptor such as that encoded by pG-N795 (ATCC 37813) or pART1/N795 (ATCC 37814).

Contains the CYC1 TATA region from S. cerevisiae (nt -178 to +49) fused at nt -178 to 3 copies of a 26 bp glucocorticoid response element (GRE) from the rat tyrosine aminotransferase gene.

A BglII/BamHI fragment with a chloramphenicol acetyltransferase coding region, its own AUG codon, and some SV40 sequences was cloned into the BamHI site of the polylinker. The other polylinker sites are 3' to this insert.

Temperature: 37.0°C