Size (kb): 7.0999999046325680

Vector: YIp358R (plasmid)

Promoters: Promoter none

Construction: YEp358R (ATCC 37739), YIp352

Marker(s):URA3,ampR

Construct size (kb): 7.099999904632568

Features: insert detection: lacZ
marker(s): ampR, URA3
promoter: none
replicon: pMB1

YI-type (integrating) shuttle vector

promoter-cloning vector

shuttle vector

The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): HindIII-2; SphI-3; PstI-3; SalI-2; XbaI-2; BamHI-2; SmaI-3; KpnI-3; SacI-3; EcoRI-2. The SacI site is not unique.

Cloning into the HindIII, SphI, PstI, SalI, or XbaI sites leads to a TAG stop codon within the downstream XbaI site of the multiple cloning region.

Restriction digests of the clone give the following sizes (kb): EcoRI-7.1; SalI-7.1; PstI-7.1; HindIII-7.1.

One of 3 promoter-cloning, YI type shuttle vectors (ATCC 37755 - 37757) with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame.

The sequence and reading frame of the multiple cloning sequence is: 5'AA GCT TGC ATG CCT GCA GGT CGA CTC TAG AGG ATC CCC GGG TAC CGA GCT CGA ATT CCC AGC TTC GAT CCC3', from nucleotide 1 of the MCS through CCC for amino acid 8 of beta-galactosidase.

Temperature: 37.0°C