Size (kb): 6.112

Vector: YCplac111 (plasmid)

Promoters: Promoter lac

Construction: pUC19

Marker(s): LEU2,ampR

Features: insert detection: lacZ'
marker(s): LEU2
marker(s): ampR
promoter: lac
replicon: ARS1
replicon: pMB1
MCS: HindIII...EcoRI
centromere: CEN4

YC-type (centromeric) shuttle vector

vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase

Restriction digests of the clone give the following sizes (kb): BglII--6.5, EcoRV--6.5, BglI--4.7, 1.7.

One of 9 shuttle vectors (ATCC 87585 - 87593) allowing lacZ detection of cloned inserts and containing the pUC19 MCS with all 10 cloning sites unique. Vectors differ in mode of replication (YE, YC or YI-type) and yeast marker (LEU2, URA3 or TRP1).

The EcoRI and KpnI restriction sites from the LEU2 gene of S. cerevisiae were removed by using oligo-directed mutagenesis.

Temperature: 37°C

component of:ATCC 87623

component of:ATCC 87638