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Vaccinia virus ts mutant

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編號:B234104

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產品名稱	Vaccinia virus ts mutant
商品貨號	B234104
Classification	Poxviridae, Orthopoxvirus
Agent	Vaccinia virus ts mutant
Strain	IHD-W Dts57 (Dales isolate 7526)
Applications	The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U14.
Positive for Mycoplasma	No
Biosafety Level	2 CDC BMBL recommends vaccination. ref Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation	derived from existing strain
Product Format	frozen
Storage Conditions	-70°C or colder
Comments	Selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. The non-permissive incubation temperature is 39.5oC. This mutant was assigned to Dales EM category H indicating that it is a composite of groups D, E and F, with foci of viroplasm, DNA paracrystals, and abberant membranes with and without spicules. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U14.
Effect on Host	Yes, Cytopathic effect: Rounding and sloughing of infected cells CPE: enlargement, plaques with rounding and eventual sloughing
Recommended Host	Recommended Host: BSC-40 (ATCC CRL-2761
Growth Conditions	Temperature: 31.0°C Duration: 1-3 days; For best results, cells should be 24 to 48 hours old and 80-90% confluent at time of infection [not 100% confluent].
Name of Depositor	S Dales, RC Condit, S Dales
Special Collection	NCRR Contract
Source	derived from existing strain
References	Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807 Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746 This mutant strain was derived by Samuel Dales from the IHD-W strain of Vaccinia (ATCC VR-1441) which in turn was derived from the IHD-J strain. cell enlargement, plaques with rounding and eventual sloughing of cells Biosafety in Microbiological and Biomedical Laboratories 4th ed.; U.S. Department of Health and Human Services ;1999. Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570

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