| 產(chǎn)品名稱 |
Influenza A virus (H1N1) |
| 商品貨號(hào) |
B233601 |
| Classification |
Orthomyxoviridae, Influenzavirus A |
| Agent |
Influenza A virus (H1N1) |
| Strain |
A/Virginia/ATCC3/2009 |
| Applications |
Emerging infectious disease research Respiratory research Influenza Research |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
This item was isolated in tissue culture. |
| Product Format |
frozen |
| Storage Conditions |
?-70°C or colder |
| Comments |
Item analyzed using Real-time RT-PCR to determine strain is of pandemic swine Influenza A origin. |
| Effect on Host |
CPE, rounded refractile cells with cell degeneration |
| Recommended Host |
MDCK (ATCC® CCL-34™) |
| Growth Conditions |
Temperature: 35°C Recommendations for Infection: Plate cells 24 hours in advance and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus diluted to provide a MOI 0.1. Adsorb 1 hour at 35°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Grow in serum free media that contains 2.0μg/ml TPCK-treated trypsin. Incubation: 2-5 days |
| Name of Depositor |
ATCC |
| Special Collection |
ATCC |
| Source |
Nasopharyngeal specimen from a patient positive for Flu A in Virginia, 2009. |
