Distribution host: Escherichia coli HB101 (ATCC 33694)

Size (kb): 5.3010001182556150

DESCRIPTION OF VECTOR:
Intact vector size: 5.301
Type of vector: plasmid
Cloning sites: SalI ClaI EcoRI PstI SmaI BamHI SpeI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS414
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
replicon: CEN6/ARSH4, ->
marker(s): TRP1, ->
terminator: CYC1, ->
promoter for expression: CYC1, <-
marker(s): ampR, <-

Vector: p414 CYC1 (plasmid)

Promoters: Promoter for expression CYC1

Construction: pRS414

Marker(s):TRP1,ampR

Construct size (kb): 5.301000118255615

Features: marker(s): TRP1
marker(s): ampR
promoter for expression: CYC1
replicon: CEN6/ARSH4
terminator: CYC1

YX-type (expression) shuttle vector

vector with low copy number

Restriction digests of the clone give the following sizes (kb): BamHI--5.6; EcoRI--5.6; SacI/XbaI--3.4, 1.7.

Contains a truncated version of the CYC1 promoter that very weak and no longer inducible because of a deletion of most of the UAS2 sequence.

Low copy number shuttle expression vector.

Temperature: 37.0°C

component of:ATCC 87669