Distribution host: Escherichia coli HB101 (ATCC 33694)

Size (kb): 7.4569997787475590

DESCRIPTION OF VECTOR:
Intact vector size: 7.457
Type of vector: plasmid
Cloning sites: XhoI SalI ClaI HindIII EcoRI SmaI BamHI SpeI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS426 (ATCC 77107)
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3, ->, 200-1300
terminator: CYC1, ->, 2004-2264
MCS: XhoI...XbaI, ->, 2265-2339
promoter for expression: ADH, <-, 2339-3839
marker(s): ampR, <-, 4958-5888
replicon: 2 micron, ->, 5987-7327

Vector: p426 ADH (plasmid)

Promoters: Promoter for expression ADH

Construction: pRS426 (ATCC 77107)

Marker(s):URA3,ampR

Construct size (kb): 7.456999778747559

Features: marker(s): URA3
marker(s): ampR
promoter for expression: ADH
replicon: 2 micron
MCS: XhoI...XbaI
terminator: CYC1

YX-type (expression) shuttle vector

Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 1.8, 1.5; EcoRI--7.4; XbaI--4.6, 2.7.

The wild type ADH promoter is active when cells are grown in glucose media but can be repressed 2-10 fold on non-fermentable carbon sources.

High copy number shuttle expression vector.

Temperature: 37.0°C

component of:ATCC 87669