Distribution host: Escherichia coli SCS1

Size (kb): 10.8000001907348600

DESCRIPTION OF VECTOR:
Intact vector size: 10.800
Type of vector: plasmid
Cloning sites: EcoRI SacI Ecl136II KpnI SmaI XmaI BamHI XbaI SalI PstI
HindIII
Polylinker sites: EcoRI SacI Ecl136II KpnI XmaI SmaI BamHI XbaI AccI SalI
HincII PstI SphI HindIII
Construction: pMMB67EH (ATCC 37622), R100.1
Host range: broad host range; Escherichia coli
Features (with orientation and position when available):
promoter: tac, ->
MCS: EcoRI...HindIII, ->
transcription terminator: rrnB
restriction site: SphI
marker(s): strR (spcR)
replicon: oriV
replicon: oriT
replicon: RSF1010
repressor gene: lacIq

Vector: pJAK12 (plasmid)

Promoters: Promoter tac

Construction: pMMB67EH (ATCC 37622), R100.1

Marker(s):strR (spcR)

Construct size (kb): 10.80000019073486

Features: marker(s): strR (spcR)
promoter: tac
replicon: RSF1010
replicon: oriT
replicon: oriV
repressor gene: lacIq
MCS: EcoRI...HindIII
restriction site: SphI
transcription terminator: rrnB

expression vector

shuttle vector

Restriction digests of the clone give the following sizes (kb): EcoRI--10.8; HindIII--10.8; SphI--9.7, 1.1.

A shuttle vector permitting autoregulated high-level expression in all gram-negative bacteria.

Compatible with common E. coli cloning vectors, as well as vectors derived from IncP and IncW plasmids.

Can be mobilized by conjugative IncP helper plasmids [e.g. pRK2013 (ATCC 37159), pUB307] into Klebsiella aerogenes, Proteus mirabilis, Pseudomonas aeruginosa, Serratia marcescens, and other gram-negative bacteria.

Belongs to a group of vectors (ATCC 37622-37623, 77287-77292) differing only in antibiotic resistance and polylinker orientation.

Temperature: 37.0°C

component of:ATCC 87055