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pYNZ22 [pYNZ22.1]
pYNZ22 [pYNZ22.1]
規格:
貨期:
編號:B231157
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pYNZ22 [pYNZ22.1]
商品貨號 B231157
Designations pYNZ22 [pYNZ22.1]
Species Homo sapiens, human
Applications
RFLPs observed under normal stringency (0.1X SSC).
Vector
Construct size (kb): 5.949999809265137
Insert
DNA: genomic
Insert lengths(kb): 1.700000047683716
Gene product: DNA Segment, single copy [D17S5]
Insert Size (kb) 1.700
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): PvuII--5.95; PstI--5.95; HindIII--5.95; EcoRI--5.95; BamHI--4.4, 1.6.
pYNZ22 (ATCC 57574) and pYNH37 (ATCC 57568) map to the same 30 kb SfiI fragment.
This clone is of the same DNA segment as pJCZ16.2 (ATCC 57658) and detects the same polymorphism.
The MspI polymorphism demonstrates codominant segregation in more than 60 families.
RFLPs observed under normal stringency (0.1X SSC).
Enzyme(s) not detecting polymorphism: BglII, EcoRI.
References

Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (pYNZ22) on chromosome 17p [D17S30]. Nucleic Acids Res. 16: 5707, 1988. PubMed: 2455284

vanTuinen P, et al. Molecular detection of microscopic and submicroscopic deletions associated with Miller-Dieker syndrome. Am. J. Hum. Genet. 43: 587-596, 1988. PubMed: 3189330

Schwartz CE, et al. Detection of submicroscopic deletions in band 17p13 in patients with the Miller-Dieker syndrome. Am. J. Hum. Genet. 43: 597-604, 1988. PubMed: 2903661

Horn GT, et al. Amplification of a highly polymorphic VNTR segment by the polymerase chain reaction. Nucleic Acids Res. 17: 2140, 1989. PubMed: 2928126

Wright EC, et al. A genetic map of human chromosome 17p. Genomics 7: 103-109, 1990. PubMed: 2335351

Odelberg SJ, et al. Characterization of eight VNTR loci by agarose gel electrophoresis. Genomics 5: 915-924, 1989. PubMed: 2574152

Barker D, et al. Gene for von Recklinghausen neurofibromatosis is in the pericentromeric region of chromosome 17. Science 236: 1100-1102, 1987. PubMed: 3107130

Yusuke Nakamura, personal communication

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