Construct size (kb): 7.800000190734863

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749

DNA: genomic

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: IGHJ
Gene name: immunoglobulin heavy polypeptide, joining region
Contains complete coding sequence?: U
Chromosome: 14; Localization: 14 q32.33
Type of DNA: genomic
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 3.4
Cross references:

Insert lengths(kb): 3.400000095367432

Gene product: immunoglobulin heavy polypeptide, joining region [IGHJ]

Alleles: A1, A2, A3, A4

Restriction digests of the clone give the following sizes (kb): BamHI--4.4, 3.4; BglII--6.4, 1.4; EcoRI--7.8; EcoRV--7.8; PstI--7.8.

This fragment originates 1.25 kb 5' to a BglII site just 5' to the J(H) sequences.

Subcloned from Ch28-6.

Enzymes(s) not detecting polymorphism: SstI, EcoRV, EcoRI, HaeIII, PvuII, PstI, TaqI, NdeII.