Construct size (kb): 3.799999952316284

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pIBI24
Intact vector size: 2.878
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI SstI KpnI XmaI SmaI BamHI XbaI SalI AccI HincII PstI
XhoI ApaI mLuI SphI HindIII
Polylinker sites: EcoRI SstI KpnI XmaI SmaI BamHI XbaI SalI AccI HincII PstI
XhoI ApaI mLuI SphI HindIII
Construction: pEMBL
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T7
replicon: pMB1, f1
terminator: none
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GZMB
Other gene symbols: CTLA1; CSPB; CCPI; CGL-1
Genomic copy number: unique
Gene name: granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated
serine esterase 1)
Contains complete coding sequence?: Y
Chromosome: 14; Localization: 14 q11.2
Tissue: T lymphocyte, activated
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 0.9
Cross references: DNA Seq. Acc.: J04071

Insert lengths(kb): 0.8999999761581421

Tissue: T lymphocyte, activated

Gene product: cytotoxic serine protease B [CCPI]

Restriction digests of the clone give the following sizes (kb): BglI--2.05, 1.35, 0.66; EcoRI--3.15, 0.92; HindIII--3.2, 0.82; PstI--3.65, 0.39; PvuII--2.7, 0.76, 0.36, 0.20; SalI--3.95.

Contains the complete coding sequence (nt 17-760).

The insert contains the following restriction sites (approximate kb from the 5' end): PvuII--0.2, 0.35; ApaI--0.22; PstI--0.35; BglI--0.4; TaqI--0.56; HindIII--0.72.

Detects genomic restriction fragments of the following sizes (approximate kb): human, BamHI--12; human, BglII--14; human, BclI--10; human, EcoRV--18; human, TaqI--3.5, 2.5; human, PstI--4.0, 2.2; mouse, PstI--4.0.