Construct size (kb): 0.0

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGX284
Intact vector size: 2.150
Type of vector: plasmid
Vector end: EcoRV
Vector end: EcoRV
Cloning sites: EcoRV
Polylinker sites:
Construction: pBR322, pC194, bacteriophage lambda, Bacillus subtilis
Host range: Bacillus subtilis; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, cmlR
replicon: pMB1
terminator: lambda
Cross references:

DNA: genomic

DESCRIPTION OF INSERT COMPONENT:
Genome: Staphylococcus aureus
Gene symbol: pra
Gene name: protein A
Contains complete coding sequence?: U
Genome: Staphylococcus aureus
Gene symbol: pra
Gene name: protein A
Contains complete coding sequence?: U
Strain: Cowan I
Type of DNA: genomic
Insert end: EcoRV
Insert end: EcoRV
Insert size (kb): 2.15
Cross references:

Insert lengths(kb): 2.150000095367432

Gene product: protein A [pra]

Restriction digests of the clone give the following sizes (kb): EcoRV--8.8, 2.15; PstI--5.6, 3.1, 2.3; HindIII--9.4, 1.6; PvuI--8.8, 2.15; BamHI--uncut.

This construct is available in two forms: in Escherichia coli as the plasmid (ATCC 39344) and integrated into the Bacillus chromosomal DNA (ATCC 39345).

The depositor suggests streaking for single colonies on L broth + 1.5% agar + 100 ug/ml ampicillin + 5 ug/ml chloramphenicol and using a single colony.

The vector contains the replicon and beta-lactamase gene from pBR322, a galactokinase gene, a CAT gene from pC194, and a transcription terminator from bacteriophage lambda. It also contains chromosomal sequences from Bacillus subtilis for integration.