Size (kb): 11.5000000000000000

Vector: pAA3H (cosmid)

Promoters: Promoter none

Construction: pBR322, lambdagal+(EcoRI/HindIII, galE'TK-cos R/L)

Marker(s):ampR,galS

Construct size (kb): 11.5

Features: insert detection: galE'TK
marker(s): ampR, galS
promoter: none
replicon: pMB1
terminator: none
enhancer: none

vector for constructing genomic libraries

vector permitting positive selection for inserts

The galE'TK genes are carried on a HindIII/HpaI fragment of approximately 3.0 kb. The galE gene is truncated at the HindIII site. Expression of the gal sequences are regulated by a promoter located between the BamHI and HindIII sites in the tet gene.

IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.

Vector for positive galactose-resistance selection of DNA cloned into the HindIII site.

Derived from pAA3.

Temperature: 30.0°C