Construct size (kb): 42.40000152587891

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pHC79
Intact vector size: 6.430
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI BamHI SalI EcaI PstI
Polylinker sites:
Construction: pBR322, Charon 4A
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, tetR
replicon: pMB1
terminator: none
Cross references: DNA Seq. Acc.: L08873

DNA: genomic

DESCRIPTION OF INSERT COMPONENT:
Genome: Saccharomyces cerevisiae
Gene name: DNA Segment
Contains complete coding sequence?: U
Chromosome: IX; Localization: IX R
Strain: AB972 (ATCC 76269)
Type of DNA: genomic
Insert end: MboI
Insert end: MboI
Insert size (kb): 36.0
Cross references:

Insert lengths(kb): 36.0

Gene product: DNA Segment

component of:ATCC 77284

Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 3.407, 5.813, 2.064, 3.028, 2.505, 3.971, 0.941, 1.169, 1.031, 1.203, 0.520, 2.072, 1.938, 3.727, 0.899, 0.564, 1.207, 0.473.

Confirmed to contain EcoRI/HindIII fragments of the following sizes (kb): 0.40, 0.49, 0.55, 0.93, 0.96, 1.1, 1.2, 2.0, 2.2, 2.6, 3.3, 3.6, 3.9, 4.2, 6.3.

IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.

Overlaps clones with ATCC number(s): 70193, 70882, 70883, 70895, 71124.