Distribution host: Escherichia coli HB101 (ATCC 33694)

Size (kb): 5.5260000228881840

DESCRIPTION OF VECTOR:
Intact vector size: 5.526
Type of vector: plasmid
Cloning sites: XhoI SalI ClaI HindIII EcoRI SmaI BamHI SpeI XbaI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: p416
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3, ->, 200-1323
terminator: CYC1, ->, 2005-2265
MCS: XhoI...XbaI, ->, 2265-2328
promoter for expression: TEF, <-, 2329-2731
marker(s): ampR, <-, 3978-4908
replicon: CEN6/ARSH4, ->, 5012-5526

Vector: p416 TEF (plasmid)

Promoters: Promoter for expression TEF

Construction: p416

Marker(s):URA3,ampR

Construct size (kb): 5.526000022888184

Features: marker(s): URA3
marker(s): ampR
promoter for expression: TEF
replicon: CEN6/ARSH4
MCS: XhoI...XbaI
terminator: CYC1

YX-type (expression) shuttle vector

vector with low copy number

Restriction digests of the clone give the following sizes (kb): BamHI--5.6; EcoRI--5.6; SacI/XbaI--5.6; SacI--uncut. The vector appears to have lost the SacI site.

Low copy number shuttle expression vector.

Temperature: 37.0°C

component of:ATCC 87669