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<i>Clostridioides difficile</i> (Prevot) Lawson et al.
<i>Clostridioides difficile</i> (Prevot) Lawson et al.
規格:
貨期:
編號:B229294
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 Clostridioides difficile (Prevot) Lawson et al.
商品貨號 B229294
Deposited As Clostridium difficile (Hall and O'Toole) Prevot
Strain Designations 1253
Application Toxin B production confirmed by cytotoxicity assay using Vero cells (ATCC CCL-81)
Enteric Research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol&reg; no
Genotype Toxinotype: 0
Type Strain no
Comments
Presence of tcdA and tcdB genes confirmed by PCR

Binary toxin gene cdtB not amplified by PCR

Toxin B production confirmed by cytotoxicity assay using Vero cells (ATCC CCL-81)

Toxinotype 0
Ribotype 054
Medium ATCC® Medium 2107: Modified Reinforced Clostridial
ATCC® Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Growth Conditions
Temperature: 37°C
Atmosphere: Anaerobic
Name of Depositor LS McClung
References

Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986

Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999

梅經理 17280875617 1438578920
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周經理 17757487661 1296385441
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沈經理 19548299266 2662369050
李經理 13626845108 972239479
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