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pCYM3
pCYM3
規格:
貨期:
編號:B228701
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pCYM3
商品貨號 B228701
Designations pCYM3
Depositors JH Camonis
Biosafety Level 1
Vector Information
Size (kb): 6.5430002212524410
Vector: pCYM3 (plasmid)
Promoters: Promoter SV40 early
Construction: pCYM2 (ATCC 37824), pTG2586
Marker(s):TRP1,ampR,URA3d
Construct size (kb): 6.543000221252441
Features: marker(s): ampR, TRP1, URA3d
promoter: SV40 early
replicon: pMB1, 2 micron
terminator: SV40
Applications
YE-type (episomal) shuttle vector
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BglII--uncut; ClaI/BamHI--3.4, 2.8, 0.5; EcoRI--6.6; BamHI/PvuII--6.0, 0.5.
URA3d is a modified URA3 gene which is not efficiently expressed and requires a large number of plasmids to complement a ura3 mutation.
Shuttle expression vector.
Permits expression in both S. cerevisiae and mouse cells.
The EcoRI, SmaI, and BamHI sites are 3' to the unique available in-phase initiation codon under control of the SV40 early promoter.
Constructed by inserting a 1.207 kb ClaI/BamHI fragment containing URA3d into pCYM2 cut with BglII and ClaI (nt 3469 and 3589 according to the pCYM0 numbering scheme).
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Camonis JH, et al. Of mice and yeast: versatile vectors which permit gene expression in both budding yeast and higher eukaryotic cells. Gene 86: 263-268, 1990. PubMed: 2108907

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