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C1-1/5 [D7S2742, UTR-9783]
C1-1/5 [D7S2742, UTR-9783]
規格:
貨期:
編號:B227981
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 C1-1/5 [D7S2742, UTR-9783]
商品貨號 B227981
Designations C1-1/5 [D7S2742, UTR-9783]
GenBank Number

M28668

Species Homo sapiens, human
Depositors JR Riordan
Vector Vector: pBluescript SK- (phagemid)
Size (kb): 2.960
Construction: pUC19
Marker(s): ampR (50 ug/ml)
Features: Insert detection: lacZ'; Promoters: T3, T7, lac; Replicons: f1, pMB1; MCS: KpnI...SacI
Sequencing primers: M13(-21), M13 reverse, T7, T3
Insert
DNA: cDNA
Insert lengths(kb): 3.25
Tissue: cystic fibrosis sweat gland
Gene product: cystic fibrosis transmembrane conductance regulator [CF]
Alleles: A1, A1, A1, A1, A2, B1, B2, A1, A1, A2, B1, B2, A2, A2, A2, A2
Insert Size (kb) 3.25
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Contains 2 internal EcoRI sites. An EcoRI digest of the plasmid results in fragments of 1.0, 1.5, and 0.75 kb from the insert.
Restriction digests of the clone give the following sizes (kb): EcoRI--2.65, 1.5, 1.1, 0.89; XbaI--6.2; PstI--6.2; BglII--5.7, 0.45; HindIII--3.2, 1.85, 0.94.
ATCC 61161 has been converted to meet the requirements of the Budapest Treaty for patent deposits. A compliance form is not needed for ATCC 61161, but is for ATCC 61160.
This probe contains a 3 bp deletion (deltaF508) in exon 10.
This clone includes exons 9 - 24 and hybridizes to genomic EcoRI fragments of 10.3, 4.3, 3.5, 2.4, 0.5, 1.8, 1.8, 3.5, 1.9, 4.5, 2.4, 1.0. 2.8, 1.8, 8.5, 2.2, and 2.3 kb.
References

Riordan JR, et al. Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA. Science 245: 1066-1073, 1989. PubMed: 2475911

J R Riordan, personal communication

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
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