Insert contains the following sites separated by (bp): EcoRI- 9- BglII- 22- PstI- 36- BglI- 148- BamHI- 29- SacI- 49- NcoI- 243- PstI- 27- NdeI- 133- BglII- 8- EcoRI. Restriction digests of the clone give the following sizes (kb): EcoRI--2.8, 0.68; PstI--3.0, 0.48; BamHI--3.4; BglI--1.5, 1.1, 0.86; SacI--3.3, 0.44. The matrix Gla gene resides on chromosome 12. |
