久欠精品国国产99国产精2021,十八禁无码精品a∨在线观看 ,久久天天躁狠狠躁夜夜躁2012,久久丫免费无码一区二区,精品视频在线观自拍自拍,久久强奷乱码老熟女,亚洲欧美另类久久久精品,精品国产福利久久久
熱門(mén)搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車(chē) 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > Perkinsus mediterraneus
最近瀏覽歷史
更多產(chǎn)品
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
Perkinsus mediterraneus
Perkinsus mediterraneus
規(guī)格:
貨期:
編號(hào):B227695
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) Perkinsus mediterraneus
商品貨號(hào) B227695
Strain Designations Ht2
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Infected oyster
Product Format frozen
Type Strain no
Medium ATCC® Medium 1886: Perkinsus broth medium
Growth Conditions
Temperature: 25.0°C
Growth condition: axenic
Cryopreservation

1.?? To achieve the best results set up cultures with several different inocula (e.g. 0.25 ml, 0.5 ml, 1.0 ml).? Harvest cultures and pool when the culture that received the lowest inoculum is at or near peak density.

2.? If the cell concentration exceeds the required level do not centrifuge, but adjust the concentration to between 2 x 106 and 2 x 107cysts/ml with fresh growth medium.? If the concentration is too low, centrifuge at 600 x g for 5 min and resuspend the pellet in the volume of fresh medium required to yield the desired concentration.

3.? While cells are centrifuging prepare a 20% (v/v) solution of sterile DMSO as follows:? Add the required volume of DMSO to a glass screw-capped test tube and place it in an ice bath.? Allow the DMSO to solidify.? Add the required volume of refrigerated medium.? Dissolve the DMSO by inverting the tube several times.?

????? *NOTE: If the DMSO solution is not prepared on ice, an exothermic reaction will occur that may precipitate certain components of the medium.

4.? Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be between 106 and 107 cells/ml and 10.0% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no less than 15 min and no longer than 30 min.

5.?? Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).

6.?? Place the vials in a controlled rate freezing unit.? From room temperature cool at -1°C/min to -40°C.? If the freezing unit can compensate for the heat of fusion, maintain rate at?????? ?-1°C/min through the heat of fusion.? At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.? Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.? (The cooling rate in this apparatus is approximately

????? -1°C/min.) ?

7. The frozen preparations are stored in either the vapor or liquid phase of a nitrogen freezer.

8.?? To establish a culture from the frozen state place an ampule in a water bath set at 35°C (2-3 min). Immerse the vial just sufficient to cover the frozen material. Do not agitate the vial.

9.?? Immediately after thawing, aseptically remove the contents of the ampule and inoculate into 10 ml of fresh ATCC medium 2684 in a T-25 tissue culture flask.? Incubate at 25-28°C.

Name of Depositor S Casas
References

Casas S, et al. Continuous culture of Perkinsus mediterraneus, a parasite of the European flat oyster Ostrea edulis, and characterization of its morphology, propagation, and extracellular proteins in vitro. J. Eukaryot. Microbiol. 55: 34-43, 2008. Pub Med: 18251801

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
主站蜘蛛池模板: 男女免费观看在线爽爽爽视频| 免费国产黄网站在线观看动图| 成av人电影在线观看| 隔壁放荡人妻bd高清| 国产精品香蕉成人网在线观看 | 婷婷色香五月综合缴缴情| 欧美牲交黑粗硬大| 亚洲综合色区中文字幕| 日日摸天天摸人人看| 亚洲理论在线a中文字幕| 日韩中文人妻无码不卡| 国产欧美日韩综合在线成| 色婷婷婷丁香亚洲综合| 亚洲国产天堂久久综合网| 最新精品国偷自产在线美女足| 国产做爰xxxⅹ久久久| 亚洲色大成成人网站久久| 成人亚洲欧美在线观看| 人人妻人人爽人人狠狠| 东京热男人av天堂| 国产欧美一区二区精品仙草咪 | 亚洲大乳高潮日本专区| 黑人巨大无码中文字幕无码 | 日韩午夜理论片 中文字幕| 亚洲一区二区三区自拍公司| 中文字幕制服丝袜人妻动态图| 性欧美videofree高清精品| 国产精品真实灌醉女在线播放 | 99视频精品国产免费观看| 中文字幕人妻无码专区| 一本一本久久a久久精品综合不卡| 亚洲色在线v中文字幕| 2021自拍偷在线精品自拍偷| 亚洲丰满熟女一区二区蜜桃| 天天爱天天做天天做天天吃中文 | 久久av在线影院| 日本久久久久亚洲中字幕| 在线亚洲综合欧美网站首页| 欧美性大战xxxxx久久久√| 国产人在线成免费视频| 亚洲午夜久久久久久噜噜噜|