Construct size (kb): 5.699999809265137

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript KS-
Intact vector size: 2.964
Type of vector: phagemid
Vector end: SalI
Vector end: SalI
Cloning sites: SacII XmaII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV
HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI
Polylinker sites: SEE COMMENTS
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: HSPCB
Other gene symbols: HSPC2
Gene name: heat shock 90kD protein 1, beta
Contains complete coding sequence?: Y
Localization: 6 p12
Tissue: fetal liver
Type of DNA: cDNA
Insert end: SalI
Insert end: SalI
Insert size (kb): 2.739
Cross references: DNA Seq. Acc.: M16660
Nucleotides 13-2524 of the insert correspond to
nucleotides 32-2543 of M16660.

Insert lengths(kb): 2.739000082015991

Tissue: fetal liver

Gene product: heat shock 90kD protein 1, beta [HSPC2]

Restriction digests of the clone give the following sizes (kb): BamHI--4.9, 0.7; HindIII--4.4, 1.4; KpnI--5.0, 0.64; PvuII--2.5, 1.7, 1.5; SalI--3.0, 2.7.

The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--2.08; BglI--0.11; EcoRI--0.97, 1.05; HindIII--1.38; KpnI--0.60; PstI--2.17, 2.30; PvuII--1.16; SacI--1.30; XhoI--2.20, 2.31.

The SalI insert contains the complete coding sequence and a poly(A) tail followed by a cloning artifact of approximately 180 bp.

The genomic sequence is available in GenBank/EMBL/DDBJ accession J04988.