Construct size (kb): 3.400000095367432

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: RAC*
Gene name: protein kinase, rac
Contains complete coding sequence?: U
Tissue: hippocampus, female, 2 years, oligo-dT and random primed
(Stratagene 936205), subtracted
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 0.4
Cross references: DNA Seq. Acc.: M78924; EST: EST01072; Primer: M13 Reverse

Insert lengths(kb): 0.4000000059604645

Tissue: hippocampus, female, 2 years, oligo-dT and random primed (Stratagene 936205), subtracted

Gene product: protein kinase, rac [RAC*]

Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.4; BamHI--3.4; HindIII--3.4; PstI--3.4.

EST01072 matches GenBank accession HUMRACPC with 100% identity over the sequence range: 108-17.

Sequenced from the M13 reverse primer.