Size (kb): 2.8959999084472660

Vector: pTZ19RBS.K (phagemid)

Promoters: Promoter T7 (phi10)

Construction: pTZ19R

Marker(s):ampR

Construct size (kb): 2.895999908447266

Features: insert detection: lacZ'
marker(s): ampR
promoter: T7 (phi10)
promoter: lacOP
replicon: f1
replicon: pMB1
MCS: EcoRI...HindIII

vector permitting construction of fusion proteins

vector permitting positive selection for inserts

vector permitting visual detection of recombinants

Restriction digests of the clone give the following sizes (kb): BamHI--2.9, EcoRI--2.9, PstI--2.9.

Cloning into the BamHI site followed by transformation into an EcoK r+ m+ host, increases the relative transformation efficiency of recombinant molecules, provided that the cloned insert is free of non-methylated EcoK sites.

Vector allowing positive selection and blue/white color detection for cloned inserts.

Vector constructed from pTZ19R by elimination of the EcoK restriction site in the ampR region and creation of a new EcoK site within the polylinker, containing a BamHI site as the central hexamer of the EcoK recognition sequence.

Temperature: 37.0°C