Construct size (kb): 5.21999979019165

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: PstI
Vector end: PstI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: FTL
Genomic copy number: unique
Gene name: ferritin, light polypeptide
Contains complete coding sequence?: Y
Chromosome: 19; Localization: 19 q13.3-q13.4
Tissue: liver
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 0.823
Cross references: DNA Seq. Acc.: M11147

Insert lengths(kb): 0.8230000138282776

Tissue: liver

Gene product: ferritin, light polypeptide [FTL]

Restriction digests of the clone give the following sizes (kb): SacI--5.0; PvuII--2.8, 2.1; NotI--5.0; HIndIII--4.5, 0.3, 0.25; PstI--4.3, 0.65, 0.25.

A PstI digest excises the insert as two fragments (bp): 593, 230. The smaller fragment contains the 5' untranslated region.

The insert contains the following restriction sites (bp from the 5' end): HindIII--569, 809; NcoI--450; NotI--372; PstI--224; PvuII--405; SacI--154.

Contains the complete coding sequence (nt 152-679).