Size (kb): 5.0409998893737790

Vector: pKSM713 (phagemid)

Promoters: Promoter for in vitro transcription T7lac

Construction: pKSM710, pSM102

Marker(s):kanR

Construct size (kb): 5.040999889373779

Features: insert detection: lacZalpha
marker(s): kanR
promoter for expression: lacUV5
promoter for in vitro transcription: T7lac
replicon: f1
replicon: pMB1
repressor gene: lacIq
MCS: BamHI...EcoRI
terminator: trpA
transcription terminator: T7
transcription terminator: fd
transcription terminator: rrnB

expression vector

vector permitting RNA synthesis in vitro

vector permitting production of single-stranded DNA

vector permitting visual detection of recombinants

Restriction digests of the clone give the following sizes (kb): BamHI--5.0; EcoRI--5.0; PstI--4.5, 0.75.

Vector contains the following restriction sites (approximate kb from nt 1): BamHI--0.34; BglII--3.47; EcoRI--0.39; KpnI--0.36, 5.02; NcoI--0.33, 2.87; PstI--2.58, 3.25; PvuI--0.51; PvuII--0.48, 3.20, 3.56, 3.90, 3.99; SacI--0.38, 5.03; SalI--2.57.

Differs from pKSM714 (ATCC 87160) only in the orientation of the pSM102 fragment containing lacIq, kanR and the trpA terminator.

Expression vector.

Temperature: 37.0°C