Size (kb): 8.0000000000000000

Vector: YEp354 (plasmid)

Promoters: Promoter none

Construction: pNM481, YEp352

Marker(s):URA3,ampR

Construct size (kb): 8.0

Features: insert detection: lacZ
marker(s): ampR, URA3
promoter: none
replicon: pMB1, 2 micron

YE-type (episomal) shuttle vector

promoter-cloning vector

shuttle vector

The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): EcoRI-1; SmaI-2; BamHI-2; SalI-2; PstI-3; HindIII-1.

Restriction digests of the clone give the following sizes (kb): EcoRI--8.0; HindIII--8.0; SmaI--8.0; BamHI--8.0; PstI--8.0; BglI--4.3, 2.3, 1.4.

One of 3 promoter-cloning, YE type shuttle vectors (ATCC 37725 - 37727) with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame.

The sequence and reading frame of the multiple cloning sequence is: 5' GAA TTC CCG GGG ATC CGT CGA CCT GCA GCC AAG CTT GCT CCC 3'. The sequence begins with the first nucleotide of the MCS and ends with the CCC for amino acid 8 of beta-galactosidase.

Temperature: 37.0°C