Size (kb): 19.2500000000000000

Vector: pSE1 (plasmid)

Construction: pYAC4DRI+Sup11,SV2NEO; Alu repeat (Blur-13)_

Marker(s):G418R,URA3,ampR,HIS3

Construct size (kb): 19.25

Features: marker(s): G418 (SV2neo)
marker(s): HIS3
marker(s): URA3
marker(s): ampR
other: Alu repeat sequence
other: telomere
replicon: ARS:TRP1
replicon: pMB1
restriction site: BamHI
restriction site: EcoRI
restriction site: NotI
restriction site: SacI
centromere: CEN4:sup11

acentric fragmentation vector

Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--9.3, 8.2, 1.8; NotI--19.3; SstI--19.3.

A Ura- yeast strain containing the desired YAC can be transformed with the 8.25 kb EcoRI-BamHI fragment of the vector, followed by selection on uracil-deficient media to form deletion variants of original clone.

The NotI and SacI sites are unique and may be used to rescue the right end of the YAC.

G418 resistance can also be used to select for transformed mammalian cells.

pSE1 (ATCC 87312) and pSE2 (ATCC 87313) differ only in the orientation of the Alu repeat sequence.

Yeast artificial chromosome (YAC) fragmentation vector allowing generation of deletions from the right end of YAC clones.

Temperature: 37.0°C