| 產(chǎn)品名稱 |
p640 |
| 商品貨號(hào) |
B224844 |
| Designations |
p640 |
| Species |
Homo sapiens, human |
| Depositors |
RA Weinberg |
| Applications |
NIH 3T3 cells were transformed with DNA from the colon adenocarcinoma SW480 cell line to produce the cell line, SW2-3. DNA from SW2-3 was used to isolate the clone. |
| Vector |
Construct size (kb): 4.940000057220459 |
| Insert |
DNA: genomic Insert lengths(kb): 0.6399999856948853 Tissue: SW480 cell line DNA transferred into NIH 3T3 cells Gene product: Kirsten rat sarcoma 2 viral (v-Ki-ras2) oncogene homolog( c-Kirsten-ras proto-oncogene, oncogene KRAS2, cellular c-Ki-ras2 proto-oncogene) [KRAS2] |
| Insert Size (kb) |
0.640 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--5.0; PstI--5.0; EcoRI/HindIII--4.4, 0.65. Encodes an intron subcloned from DNA of a secondary transformant cloned into the EcoRI site of Charon 16A. This probe contains a unique sequence derived from a 2.6 kb EcoRI fragment that is present in all transfectants containing the SW480 oncogene. NIH 3T3 cells were transformed with DNA from the colon adenocarcinoma SW480 cell line to produce the cell line, SW2-3. DNA from SW2-3 was used to isolate the clone. |
| References |
McCoy MS, et al. Characterization of a human colon/lung carcinoma oncogene. Nature 302: 79-81, 1983. PubMed: 6298638
Janssen JW, et al. Ras gene mutations in acute and chronic myelocytic leukemias, chronic myeloproliferative disorders, and myelodysplastic syndromes. Proc. Natl. Acad. Sci. USA 84: 9228-9232, 1987. PubMed: 3122217
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