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16C8m [pET-3cT]
16C8m [pET-3cT]
規格:
貨期:
編號:B224503
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 16C8m [pET-3cT]
商品貨號 B224503
Designations 16C8m [pET-3cT]
Species Mus musculus, mouse
Depositors ET Cocuzzi, SE Walther
Applications
in another host, produces protein tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor)
Vector
Construct size (kb): 5.599999904632568
Insert
DNA: cDNA
Insert lengths(kb): 0.6000000238418579
Tissue: embryo fibroblasts
Gene product: tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor) [Timp]
Target Gene: tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor)
Insert Size (kb) 0.600
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/NdeI--5.0, 0.6; PstI--3.9, 1.7; EcoRV--4.8, 0.55, 0.1; BamHI--5.6; MboII--1.65, 0.9, 0.75, 0.7, 0.6, 0.27, 0.1, +smaller.
The insert contains the following restriction sites (bp from the 5' end): HaeI--4; EcoRV--177; PstI--304; XmnI--251.
Constructed by modifying codon 23 from AAG to ATG, changing a StuI site to a NdeI site. The insert extends 49 bp 3' of the termination codon to the BamHI site.
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Cocuzzi ET, et al. Expression and purification of mouse TIMP-1 from E. coli. FEBS Lett. 307: 375-378, 1992. PubMed: 1322839

Enzo T Cocuzzi, personal communication

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
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