Triple filtered through 0.1 μm filters. Each lot of fetal bovine serum is tested for sterility and for the ability to support the growth of several different cell lines using both sequential growth curves and plating efficiencies. Fetal bovine serum is manufactured from fetal bovine blood collected in USDA-inspected abattoirs located in the United States.

Sterility tests are performed on each lot of fetal bovine serum using current USP and EP methods. Specific tests for contaminants are listed below in the Specification Certificate.  ATCC also checks each lot for its ability to support the growth of several different cell lines. Additionally, this lot of serum is tested for its ability to support the growth and differentiation of embryonic stem (ES) cells. Plating efficiency, colony morphology, and the expression of at least five markers of differentiation are determined for undifferentiated cells and cells induced to form embryoid bodies (EB) through full differentiation. The results are compared with cells grown with a control lot of serum.

Origin
This fetal bovine serum is manufactured from fetal bovine blood collected in USDA-inspected abattoirs located in the United States.